PMO

A Phosphorodiamidate Morpholino Oligomer (PMO) is a synthetic nucleic acid analog used primarily in molecular biology and therapeutic applications. PMOs feature a backbone of phosphorodiamidate linkages, which replace the standard phosphodiester linkages found in natural nucleic acids. This modification enhances chemical stability and provides resistance to enzymatic degradation. The term "Morpholino" refers to the six-membered ring structure that substitutes the sugar component of nucleotides in conventional RNA or DNA. This ring is integrated into the backbone, contributing to the PMO’s overall stability and specificity

PMOs are designed to bind to specific RNA sequences through complementary base pairing. By doing so, they can modulate or inhibit the expression of targeted RNA molecules, making them particularly useful for gene silencing and antisense therapy. Due to their stability and specificity, PMOs are valuable tools in research for studying gene function and have potential therapeutic applications for treating various genetic disorders, viral infections, and other conditions involving aberrant gene expression

In summary, PMOs are synthetic oligonucleotides with a modified backbone structure that enhances their stability and specificity, making them effective tools in both research and therapeutic contexts

Genelixir can provide the following types of Morpholino oligos:

Custom Specified Morpholino Oligos: These are tailored to specific sequences or targets that you need for your research. You can provide the exact nucleotide sequence you want to target.

Control Oligos: These are typically designed to have a sequence that does not target any specific mRNA. They serve as negative controls in experiments to help differentiate between specific and non-specific effects

Morpholino Oligos with Modifications on the 3' and/or 5' Ends: Morpholino oligos with modifications on the 3' and/or 5' ends can affect their stability, binding affinity, or delivery. When modifying Morpholino oligos for conjugation with biologically active cargo such as fluorescent dyes, drugs, or peptides using a suitable linker at the 5' and/or 3' ends is a versatile approach. The linker enables various chemical reactions, including Michael addition, Click conjugation, and amide coupling, to attach different cargo molecules.

5'-end Modifications of Morpholino oligo